MAPK13 antibody (C-Term)

Catalog No. ABIN3043610

The Rabbit Polyclonal anti-MAPK13 antibody has been validated for WB and IHC. It is suitable to detect MAPK13 in samples from Human, Mouse and Rat.

Quick Overview for MAPK13 antibody (C-Term) (ABIN3043610)

Target: MAPK13 (Mitogen-Activated Protein Kinase 13 (MAPK13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAPK13 Antibody

Binding Specificity: AA 332-365, C-Term

Purpose: Anti-SAPK4/MAPK13 Antibody Picoband®

Sequence: KLTVDEWKQH IYKEIVNFSP IARKDSRRRS GMKL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SAPK4/MAPK13 Antibody Picoband® (ABIN3043610). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SAPK4 , different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Goedert, M., Cuenda, A., Craxton, M., Jakes, R., Cohen, P. Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6), comparison of its substrate specificity with that of other SAP kinases. EMBO J. 16: 3563-3571, 1997. 2. Kumar, S., McDonnell, P. C., Gum, R. J., Hand, A. T., Lee, J. C., Young, P. R. Novel homologues of CSBP/p38 MAP kinase: activation, substrate specificity and sensitivity to inhibition by pyridinyl imidazoles. Biochem. Biophys. Res. Commun. 235: 533-538, 1997. 3. Wang, X. S., Diener, K., Manthey, C. L., Wang, S., Rosenzweig, B., Bray, J., Delaney, J., Cole, C. N., Chan-Hui, P.-Y., Mantlo, N., Lichenstein, H. S., Zukowski, M., Yao, Z. Molecular cloning and characterization of a novel p38 mitogen-activated protein kinase. J. Biol. Chem. 272: 23668-23674, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAPK13

Target: MAPK13 (Mitogen-Activated Protein Kinase 13 (MAPK13))

Alternative Name: MAPK13

Background:

Synonyms: Mitogen-activated protein kinase 13,MAP kinase 13,MAPK 13,2.7.11.24,Mitogen-activated protein kinase p38 delta,MAP kinase p38 delta,Stress-activated protein kinase 4,MAPK13,PRKM13, SAPK4,

Tissue Specificity: Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. .

Background: MAPK13 (Mitogen-Activated Protein Kinase 13), also called p38-DELTA or Stress-Activated Protein Kinase 4 (SAPK4), is an enzyme that in humans is encoded by the MAPK13 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.

Molecular Weight: 42 kDa

Gene ID: 5603

UniProt: O15264

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Hepatitis C, BCR Signaling, S100 Proteins