Annexin a1 antibody (N-Term)

Catalog No. ABIN3043680

Cited in 3+ publications. This anti-Annexin a1 antibody is a Rabbit Polyclonal antibody detecting Annexin a1 in WB, IHC, FACS, ICC and IF. Suitable for Human and Monkey.

Quick Overview for Annexin a1 antibody (N-Term) (ABIN3043680)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Annexin a1 Antibody

Binding Specificity: AA 3-24, N-Term

Purpose: Anti-Annexin A1/ANXA1 Antibody

Sequence: MVSEFLKQAW FIENEEQEYV QT

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Annexin A1/ANXA1 Antibody (ABIN3043680). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Annexin a1 antibody (ABIN3043680)

Zhang, Wu, Zha, Zhao, Feng: "The prognostic value and clinicopathological features of sarcomatoid differentiation in patients with renal cell carcinoma: a systematic review and meta-analysis." in: Cancer management and research, Vol. 10, pp. 1687-1703, (2018) (PubMed).

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Mohamed, Idris, Ahmed, Åstrøm, Mustafa, Ibrahim, Mustafa: "Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: a cross-sectional study." in: BMC oral health, Vol. 15, pp. 86, (2015) (PubMed).

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: ANXA1

Background:

Synonyms: Annexin A1,Annexin I,Annexin-1,Calpactin II,Calpactin-2,Chromobindin-9,Lipocortin I,Phospholipase A2 inhibitory protein,p35,ANXA1,ANX1, LPC1,

Tissue Specificity: Widely expressed, with highest expression in brain. .

Background: Annexin I, also known as lipocortin I (Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins (I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.

Sequence Similarities: Belongs to the annexin family.

Molecular Weight: 39 kDa

UniProt: P04083

Pathways: Hormone Transport