TIMP3 antibody (C-Term)

Catalog No. ABIN3043694

The Rabbit Polyclonal anti-TIMP3 antibody has been validated for WB and ELISA. It is suitable to detect TIMP3 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for TIMP3 antibody (C-Term) (ABIN3043694)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TIMP3 Antibody

Binding Specificity: AA 198-211, C-Term

Purpose: Anti-Metalloproteinase inhibitor 3 TIMP3 Antibody Picoband®

Sequence: WAPPDKSIIN ATDP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Metalloproteinase inhibitor 3 TIMP3 Antibody (ABIN3043694). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP3, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: TIMP3 TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory)". 2. Apte, S. S., Mattei, M.-G., Olsen, B. R. : Cloning of the cDNA encoding human tissue inhibitor of metalloproteinases-3 (TIMP-3) and mapping of the TIMP3 gene to chromosome 22. Genomics 19: 86-90, 1994. 3. Qi JH, Ebrahem Q, Moore N, Murphy G, Claesson-Welsh L, Bond M, Baker A, Anand-Apte B (Apr 2003). "A novel function for tissue inhibitor of metalloproteinases-3 (TIMP3): inhibition of angiogenesis by blockage of VEGF binding to VEGF receptor-2". Nat Med 9 (4): 407-15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TIMP3 antibody (ABIN3043694)

Guan, Zhang, Song, Dai: "Promoter methylation and expression of TIMP3 gene in gastric cancer." in: Diagnostic pathology, Vol. 8, pp. 110, (2014) (PubMed).

Bai, Yi, Li, Sui: "Clinicopathologic significance of BAG1 and TIMP3 expression in colon carcinoma." in: World journal of gastroenterology, Vol. 13, Issue 28, pp. 3883-5, (2007) (PubMed).

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3

Background:

Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,

Background: Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. It is mapped to 22q12.1-q13.2. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of theextracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.

Sequence Similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Molecular Weight: 91 kDa

UniProt: P35625