HRH3 antibody (C-Term)

Catalog No. ABIN3043704

The Rabbit Polyclonal anti-HRH3 antibody (ABIN3043704) specifically detects HRH3 in WB. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for HRH3 antibody (C-Term) (ABIN3043704)

Target: HRH3 (Histamine Receptor H3 (HRH3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRH3 Antibody

Binding Specificity: C-Term

Purpose: Anti-Histamine H3 receptor HRH3 Antibody

Sequence: CPQKLKIQPH SSLEHCWK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by three amino acids.

Characteristics: Anti-Histamine H3 receptor HRH3 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3, different from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: HRH3

Background:

Background: The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22 % and 21.4 % amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Gene Full Name: histamine receptor H3

Molecular Weight: 49 kDa

Gene ID: 11255

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour