ATG14 antibody (N-Term)

Catalog No. ABIN3043721

The Rabbit Polyclonal anti-ATG14 antibody has been validated for WB, IHC, FACS, IF and ICC. It is suitable to detect ATG14 in samples from Human and Rat.

Quick Overview for ATG14 antibody (N-Term) (ABIN3043721)

Target: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATG14 Antibody

Binding Specificity: AA 70-101, N-Term

Purpose: Anti-ATG14L Antibody Picoband®

Sequence: RDRERFIDKK ERLSRLKSKQ EEFQKEVLKA ME

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ATG14L Antibody Picoband® (ABIN3043721). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATG14L, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, Zhou Q, Wilz LM, Li J, Vivona S, Pfuetzner RA, Brunger AT, Zhong Q. ""ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes"". Nature, 2015 Apr 23.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATG14

Target: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Alternative Name: ATG14

Background:

Synonyms: Beclin 1-associated autophagy-related key regulator ,Barkor ,Autophagy-related protein 14-like protein ,Atg14L ,ATG14 ,ATG14L , KIAA0831,

Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Background: ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.

Sequence Similarities: Belongs to the ATG14 family.

Molecular Weight: 59 kDa

Gene ID: 22863

Pathways: Autophagy