Arylsulfatase A antibody (C-Term)

Catalog No. ABIN3043722

The Rabbit Polyclonal anti-Arylsulfatase A antibody (ABIN3043722) specifically detects Arylsulfatase A in WB, IHC, ICC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Arylsulfatase A antibody (C-Term) (ABIN3043722)

Target: Arylsulfatase A (ARSA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Arylsulfatase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Arylsulfatase A Antibody

Binding Specificity: C-Term

Purpose: Anti-ARSA Antibody

Sequence: QALKQLQLLK AQLDAAVTFG PSQVARGED

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by six amino acids.

Characteristics: Anti-ARSA Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA, different from the related mouse sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Arylsulfatase A

Target: Arylsulfatase A (ARSA)

Alternative Name: ARSA

Background:

Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Gene Full Name: arylsulfatase A

Sequence Similarities: Belongs to the sulfatase family.

Molecular Weight: 54 kDa

Gene ID: 410

UniProt: P15289