ALOX15 antibody (AA 2-337)

Catalog No. ABIN3043726

Product Details anti-ALOX15 Antibody

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Binding Specificity: AA 2-337

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® (ABIN3043726). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. Funk CD, Funk LB, FitzGerald GA, Samuelsson B (May 1992)."Characterization of human 12-lipoxygenase genes".Proceedings of the National Academy of Sciences of the United States of America 89 (9): 3962-6. 2. Kelavkar, U. P., Badr, K. F. Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. Proc. Nat. Acad. Sci. 96: 4378-4383, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: ALOX15 (ALOX15 Products)

Background:

Synonyms: Arachidonate 15-lipoxygenase,15-LOX,15-LOX-1,1.13.11.33,12/15-lipoxygenase,Arachidonate 12-lipoxygenase, leukocyte-type,12-LOX,1.13.11.31,Arachidonate omega-6 lipoxygenase,ALOX15,LOG15,

Tissue Specificity: Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells. .

Background: Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in ionally opposite manners.

Sequence Similarities: Belongs to the lipoxygenase family.

Molecular Weight: 75 kDa

Gene ID: 246

UniProt: P16050

Pathways: Regulation of Actin Filament Polymerization