ADRBK2 antibody (C-Term)

Catalog No. ABIN3043729

This Rabbit Polyclonal antibody specifically detects ADRBK2 in WB, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for ADRBK2 antibody (C-Term) (ABIN3043729)

Target: ADRBK2 (Adrenergic, Beta, Receptor Kinase 2 (ADRBK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRBK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ADRBK2 Antibody

Binding Specificity: C-Term

Purpose: Anti-GRK3/ADRBK2 Antibody

Sequence: ESDPEFVQWK KELNETFKEA QRLLRRAPKF LNKPR

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by five amino acids.

Characteristics: Anti-GRK3/ADRBK2 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GRK3, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ADRBK2

Target: ADRBK2 (Adrenergic, Beta, Receptor Kinase 2 (ADRBK2))

Alternative Name: ADRBK2

Background:

Background: Beta-adrenergic receptor kinase 2  (beta-ARK-2), also known as G-protein-coupled receptor kinase 3  (GRK3), is an enzyme that in humans is encoded by the ADRBK2 gene. The human ADRBK2 gene is located on 22q11. The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.

Gene Full Name: G protein-coupled receptor kinase 3

Molecular Weight: 80 kDa

Gene ID: 157

UniProt: P35626

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling