alpha 1 Adrenergic Receptor antibody (C-Term)

Catalog No. ABIN3043732

This Rabbit Polyclonal antibody specifically detects alpha 1 Adrenergic Receptor in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for alpha 1 Adrenergic Receptor antibody (C-Term) (ABIN3043732)

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 1 Adrenergic Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-alpha 1 Adrenergic Receptor Antibody

Binding Specificity: AA 335-373, C-Term

Purpose: Anti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband®

Sequence: KAFQNVLRIQ CLCRKQSSKH ALGYTLHPPS QAVEGQHKD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband® (ABIN3043732). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ADRA1A, different from the related mouse and rat sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Hirasawa, A., Horie, K., Tanaka, T., Takagaki, K., Murai, M., Yano, J., Tsujimoto, G.Cloning, functional expression and tissue distribution of human cDNA for the alpha-1C-adrenergic receptor. Biochem. Biophys. Res. Commun. 195: 902-909, 1993. 2. Langer SZ (1998). "Nomenclature and state of the art on alpha1-adrenoceptors". Eur. Urol. 33 Suppl 2: 2-6. 3. Weinberg, D. H., Trivedi, P., Tan, C. P., Mitra, S., Perkins-Barrow, A., Borkowski, D., Strader, C. D., Bayne, M. Cloning, expression and characterization of human alpha adrenergic receptors alpha-1A, alpha-1B, and alpha-1C. Biochem. Biophys. Res. Commun. 201: 1296-1304, 1994.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for alpha 1 Adrenergic Receptor

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Alternative Name: ADRA1A

Background:

Synonyms: Alpha-1A adrenergic receptor,Alpha-1A adrenoreceptor,Alpha-1A adrenoceptor,Alpha-1C adrenergic receptor,Alpha-adrenergic receptor 1c,ADRA1A,ADRA1C,

Tissue Specificity: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .

Background: ADRA1A, also known as alpha-1A adrenergic receptor, is an alpha-1 adrenergic receptor, and also denotes the human gene encoding it. This gene is mapped to 8p21.2. Alpha-1-adrenergic receptors are G protein-coupled transmembrane receptors that mediate actions in the sympathetic nervous system through the binding of the catecholamines, epinephrine and norepinephrine. It has been found that ADRA1A transcripts in heart, brain, liver, and prostate. ADRA1A is the predominant ADRA1 subtype in liver and heart, and it can mediate the contraction of prostate smooth muscle.

Molecular Weight: 51 kDa

Gene ID: 148

UniProt: P35348

Pathways: AMPK Signaling