ABCG2 antibody (N-Term)

Catalog No. ABIN3043775

There are 6+ publications for this product available. The Rabbit Polyclonal anti-ABCG2 antibody is suitable to detect ABCG2 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for ABCG2 antibody (N-Term) (ABIN3043775)

Target: ABCG2 (ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ABCG2 Antibody

Binding Specificity: N-Term

Purpose: Anti-BCRP/ABCG2 Antibody

Sequence: RENLQFSAAL RLATTMTNHE KNERINRVIQ EL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by five amino acids,and from the related rat sequence by eight amino acids.

Characteristics: Anti-BCRP/ABCG2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG2, different from the related mouse sequence by five amino acids, and from the related rat sequence by eight amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P)and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-ABCG2 antibody (ABIN3043775)

Chen, Wei, Cheng, Xie, Wang, Yi, Tian, Liu, Wang, Zhang: "Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells." in: Oncology letters, Vol. 15, Issue 1, pp. 1255-1262, (2018) (PubMed).

Zhu, Li, Luo, Wang, Li, Xie, Gao, Song, Su, Liang et al.: "Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related ..." in: Journal of translational medicine, Vol. 13, pp. 356, (2016) (PubMed).

Wang, Zhao, Wu, He, Qu, Zhao, Zhao, Li, Wang: "Mechanistic analysis of taxol-induced multidrug resistance in an ovarian cancer cell line." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 14, Issue 9, pp. 4983-8, (2013) (PubMed).

Li, Wu, Wei, Tian: "Characterization of side population cells isolated from the gastric cancer cell line SGC-7901." in: Oncology letters, Vol. 5, Issue 3, pp. 877-883, (2013) (PubMed).

Duan, Mansour, Zhang, Deng, Chen, Wang, Pan, Zhao: "Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles." in: International journal of pharmaceutics, Vol. 426, Issue 1-2, pp. 193-201, (2012) (PubMed).

Qiao, Johnson, Chen, Li, Tao, Gao: "Disclosure of a stem cell phenotype in an oral squamous cell carcinoma cell line induced by BMP-4 via an epithelial-mesenchymal transition." in: Oncology reports, Vol. 26, Issue 2, pp. 455-61, (2011) (PubMed).

Target Details for ABCG2

Target: ABCG2 (ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2))

Alternative Name: ABCG2

Background:

Background: ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded that ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

Gene Full Name: ATP binding cassette subfamily G member 2 (JR blood group)

Molecular Weight: 75-80 kDa

Gene ID: 9429

UniProt: Q9UNQ0

Pathways: Transition Metal Ion Homeostasis