ATP2A1/SERCA1 antibody (N-Term)

Catalog No. ABIN3043793

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATP2A1/SERCA1 antibody is suitable to detect ATP2A1/SERCA1 in samples from Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for ATP2A1/SERCA1 antibody (N-Term) (ABIN3043793)

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A1/SERCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATP2A1/SERCA1 Antibody

Binding Specificity: AA 1-32, N-Term

Purpose: Anti-SERCA2 ATPase/ATP2A2 Antibody

Sequence: MENAHTKTVE EVLGHFGVNE STGLSLEQVK KL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SERCA2 ATPase/ATP2A2 Antibody (ABIN3043793). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA2 ATPase, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ATP2A1/SERCA1 antibody (ABIN3043793)

Guo, Zeng, Zhang, Zhang, Li, Wu, Guan, Liu, Zhang, Li, Wan: "Extracellular matrix of mechanically stretched cardiac fibroblasts improves viability and metabolic activity of ventricular cells." in: International journal of medical sciences, Vol. 10, Issue 13, pp. 1837-45, (2013) (PubMed).

Target Details for ATP2A1/SERCA1

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Alternative Name: ATP2A1

Background:

Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2,SERCA2,SR Ca (2+)-ATPase 2,3.6.3.8,Calcium pump 2,Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform,Endoplasmic reticulum class 1/2 Ca (2+) ATPase,ATP2A2,ATP2B,

Tissue Specificity: Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .

Background: SERCA2, also called ATP2A2 or ATP2B, encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca (2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped to 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression. SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles, germinative and mature cells of sebaceous glands, secretory coil and duct of eccrine glands, apocrine gland cells, and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells.

Sequence Similarities: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Molecular Weight: 115 kDa

Gene ID: 488

UniProt: P16615

Pathways: Ribonucleoside Biosynthetic Process