CLOCK antibody (N-Term)

Catalog No. ABIN3043813

This Rabbit Polyclonal antibody specifically detects CLOCK in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CLOCK antibody (N-Term) (ABIN3043813)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLOCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-CLOCK Antibody

Binding Specificity: N-Term

Purpose: Anti-KAT13D/CLOCK Antibody

Sequence: QKSIDFLRKH KEITAQSDAS EIRQDWKPTF LSNEE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by one amino acid,and identical to the related rat sequence.

Characteristics: Anti-KAT13D/CLOCK.Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KAT13D/CLOCK, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Alternative Name: CLOCK

Background:

Background: Clock (Circadian Locomotor Output Cycles Kaput) is also known as KAT13D. The protein encoded by this gene plays a central role in the regulation of circadian rhythms. This protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. And the encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.

Gene Full Name: clock circadian regulator

Molecular Weight: 110 kDa

Gene ID: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism