HLA-C antibody (C-Term)

Catalog No. ABIN3043843

Product Details anti-HLA-C Antibody

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Binding Specificity: AA 38-62, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-C antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for MHC class I antigen(HLA-C) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: EYWDRETQKY KRQAQADRVN LRKLR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MHC class I antigen(HLA-C) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: major histocompatibility complex, class I, C
Protein Name: MHC class I antigen

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C(38-62aa EYWDRETQKYKRQAQADRVNLRKLR).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for HLA-C is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HLA-C

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Alternative Name: HLA-C (HLA-C Products)

Synonyms: D6S204 antibody, HLA-JY3 antibody, HLC-C antibody, PSORS1 antibody, major histocompatibility complex, class I, C antibody, HLA-C antibody

Background: HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

Synonyms: 1C07_HUMAN antibody|Cw-7 alpha chain antibody|D6S204 antibody|HLA class I histocompatibility antigen antibody|HLA class I histocompatibility antigen, C alpha chain antibody|HLA class I histocompatibility antigen, Cw-1 alpha chain antibody|HLA-C antibodyHLA-C histocompatibility type antibody|HLA-Cw antibody|HLA-JY3 antibody|HLAC antibody|HLC-C antibody|human leukocyte antigen-C alpha chain antibody|major histocompatibility antigen HLA-C antibody|major histocompatibility complex, class I, C antibody|MHC class I antigen antibody|MHC class I antigen Cw 1 antibody|MHC class I antigen Cw*7 antibody|MHC class I antigen heavy chain HLA-C antibody|PSORS1 antibody

Gene ID: 3107

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process