P2RX5 antibody (AA 333-422)

Catalog No. ABIN3043894

Product Details anti-P2RX5 Antibody

Target: P2RX5 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 5 (P2RX5))

Binding Specificity: AA 333-422

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for P2X purinoceptor 5(P2RX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for P2X purinoceptor 5(P2RX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: purinergic receptor P2X, ligand gated ion channel, 5
Protein Name: P2X purinoceptor 5

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human P2X5 recombinant protein (Position: D333-T422). Human P2X5 shares 42% amino acid (aa) sequence identity with rat P2X5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for P2X5 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-P2RX5 antibody (ABIN3043894)

Meng, Zheng, Yan, Li, Fang, Li: "P2X2 and P2X5 Receptors Mediate Bladder Hyperesthesia in ICC in Female Overactive Bladder." in: Cell biochemistry and biophysics, Vol. 72, Issue 2, pp. 375-83, (2016) (PubMed).

Target Details for P2RX5

Target: P2RX5 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 5 (P2RX5))

Alternative Name: P2RX5 (P2RX5 Products)

Synonyms: P2RX5 antibody, LRH-1 antibody, P2X5 antibody, P2X5R antibody, P2x5 antibody, purinergic receptor P2X 5 antibody, purinergic receptor P2X, ligand-gated ion channel, 5 antibody, P2RX5 antibody, P2rx5 antibody

Background: P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. It has been identified that the P2RX5 gene within a BAC contig covering chromosome 17p13. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Quantitative dot blots revealed that P2X5R expression is highest in brain and immune system and is developmentally regulated.

Synonyms: ATP receptor antibody|ATP receptor subunit antibody|Ionotropic ATP receptor P2X5 antibody|LRH 1 antibody|LRH1 antibody|Lymphoid restricted histocompatibility antigen 1 antibody|MGC47755 antibody|P2rx5 antibody|P2RX5_HUMAN antibody|P2X purinoceptor 5 antibody|P2X5 antibody|P2X5R antibody|Purinergic receptor antibody|Purinergic receptor P2X ligand gated ion channel 5 antibody|Purinergic receptor P2X5 antibody

Gene ID: 5026

UniProt: Q93086