POMC antibody (Middle Region)

Catalog No. ABIN3043901

There are 2+ publications for this product available. The Rabbit Polyclonal anti-POMC antibody is suitable to detect POMC in samples from Human, Mouse and Rat. It has been validated for IHC.

Quick Overview for POMC antibody (Middle Region) (ABIN3043901)

Target: POMC (Proopiomelanocortin (POMC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-POMC Antibody

Binding Specificity: Middle Region

Purpose: Anti-ACTH/POMC Antibody

Sequence: SYSMEHFRWG KPVGKKRRPV KVYPNGAEDE SAEAFPLEF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by two amino acids.

Characteristics: Anti-ACTH/POMC Antibody. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ACTH, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-POMC antibody (ABIN3043901)

Huo, Liu, Wang, Zhang, Wang, Yang, Sun, Xu: "Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury." in: Neural regeneration research, Vol. 7, Issue 22, pp. 1729-35, (2015) (PubMed).

Chen, Wang, Zhao, Zhang, Huang: "Effects of the extract of a Chinese herb Tripterygium wilfordii hook f on rat pituitary gland." in: The American journal of Chinese medicine, Vol. 33, Issue 6, pp. 945-55, (2006) (PubMed).

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background:

Background: Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. ACTH stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. This gene can influence steroid hormone secretion by both rapid short-term mechanisms that take place within minutes and slower long-term actions. Besides, ACTH also enhances transcription of mitochondrial genes that encode for subunits of mitochondrial oxidative phosphorylation systems.

Gene Full Name: proopiomelanocortin

Sequence Similarities: Belongs to the POMC family.

Molecular Weight: 30 kDa

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour