RAB13 antibody (C-Term)

Catalog No. ABIN3043916

The Rabbit Polyclonal anti-RAB13 antibody has been validated for WB, FACS, IF and ICC. It is suitable to detect RAB13 in samples from Human.

Quick Overview for RAB13 antibody (C-Term) (ABIN3043916)

Target: RAB13 (RAB13, Member RAS Oncogene Family (RAB13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB13 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAB13 Antibody

Binding Specificity: AA 121-150, C-Term

Purpose: Anti-RAB13 Antibody Picoband®

Sequence: NKCDMEAKRK VQKEQADKLA REHGIRFFET

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB13 Antibody Picoband® (ABIN3043916). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB13, different from the related mouse and rat sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: RAB13 RAB13, member RAS oncogene family". 2. Zahraoui A, Joberty G, Arpin M, Fontaine JJ, Hellio R, Tavitian A, Louvard D (Feb 1994). "A small rab GTPase is distributed in cytoplasmic vesicles in non polarized cells but colocalizes with the tight junction marker ZO-1 in polarized epithelial cells". J Cell Biol 124 (1-2): 101-15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB13

Target: RAB13 (RAB13, Member RAS Oncogene Family (RAB13))

Alternative Name: RAB13

Background:

Synonyms: Ras-related protein Rab-13,Cell growth-inhibiting gene 4 protein,RAB13,GIG4,

Tissue Specificity: Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.

Background: Ras-related protein Rab-13 is a protein that in humans is encoded by the RAB13 gene. This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12.

Molecular Weight: 23 kDa

Gene ID: 5872

UniProt: P51153

Pathways: Cell-Cell Junction Organization