SLC9A1 antibody (AA 543-815)

Catalog No. ABIN3043932

This anti-SLC9A1 antibody is a Rabbit Polyclonal antibody detecting SLC9A1 in WB and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for SLC9A1 antibody (AA 543-815) (ABIN3043932)

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC9A1 Antibody

Binding Specificity: AA 543-815

Purpose: Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband® (ABIN3043932). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Fliegel L, Dyck JR, Wang H, Fong C, Haworth RS (August 1993). "Cloning and analysis of the human myocardial Na+/H+ exchanger". Mol. Cell. Biochem. 125 (2): 137-43. 2. Mattei MG, Sardet C, Franchi A, Pouysségur J (1988). "The human amiloride-sensitive Na+/H+ antiporter: localization to chromosome 1 by in situ hybridization". Cytogenet. Cell Genet. 48 (1): 6-8. 3. Denker, S. P., Huang, D. C., Orlowski, J., Furthmayr, H., Barber, D. L. binding of the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Molec. Cell 6: 1425-1436, 2000. 4. Dudley, C. R. K., Giuffra, L. A., Tippett, P., Kidd, K. K., Reeders, S. T. The Na+/H+ antiporter: a 'melt' polymorphism allows regional mapping to the short arm of chromosome 1. Hum. Genet. 86: 79-83,1990.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Alternative Name: SLC9A1

Background:

Synonyms: Sodium/hydrogen exchanger 1,APNH,Na (+)/H (+) antiporter, amiloride-sensitive,Na (+)/H (+) exchanger 1,NHE-1,Solute carrier family 9 member 1,SLC9A1,APNH1, NHE1,

Tissue Specificity: Kidney and intestine.

Background: NHE1, also known as SLC9A1, is an isoform of sodium-hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Sequence Similarities: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Molecular Weight: 91 kDa

Gene ID: 6548

UniProt: P19634

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport