PLK2 antibody (C-Term)

Catalog No. ABIN3043982

The Rabbit Polyclonal anti-PLK2 antibody is suitable to detect PLK2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC, FACS and IHC (fro).

Quick Overview for PLK2 antibody (C-Term) (ABIN3043982)

Target: PLK2 (Polo-Like Kinase 2 (PLK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PLK2 Antibody

Binding Specificity: AA 666-685, C-Term

Purpose: Anti-PLK2 Antibody

Sequence: SSELKNRMEY ALNMLLQRCN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PLK2 Antibody (ABIN3043982). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PLK2, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PLK2

Target: PLK2 (Polo-Like Kinase 2 (PLK2))

Alternative Name: PLK2

Background:

Synonyms: Serine/threonine-protein kinase PLK2,2.7.11.21,Polo-like kinase 2,PLK-2,hPlk2,Serine/threonine-protein kinase SNK,hSNK,Serum-inducible kinase,PLK2,SNK,

Tissue Specificity: Expressed at higher level in the fetal lung, kidney, spleen and heart. .

Background: Polo-like kinase2, also known as SNK is an enzyme that in humans is encoded by the PLK2 gene. PLK2 is a member of the "polo" family of serine/threonine protein kinases that have a role in normal cell division. The International Radiation Hybrid Mapping Consortium mapped the SNK gene to chromosome 5. SNK involved in synaptic plasticity, centriole duplication and G1/S phase transition. This gene plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signalin: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome.

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Molecular Weight: 78 kDa