Transglutaminase 2 antibody (C-Term)

Catalog No. ABIN3043989

Product Details anti-Transglutaminase 2 Antibody

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Binding Specificity: AA 399-418, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Protein-glutamine gamma-glutamyltransferase 2(TGM2) detection. Tested with WB, IHC-P, ICC in Human,Rat.

Sequence: ADVVDWIQQD DGSVHKSINR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein-glutamine gamma-glutamyltransferase 2(TGM2) detection. Tested with WB, IHC-P, ICC in Human,Rat.
Gene Name: transglutaminase 2
Protein Name: Protein-glutamine gamma-glutamyltransferase 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TGM2 (399-418aa ADVVDWIQQDDGSVHKSINR), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2 (TGM2 Products)

Background: Tissue transglutaminase, also called TGC, is a 78- kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization(FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.

Synonyms: ALPHA SUBUNIT antibody|C polypeptide antibody|EC 2.3.2.13 antibody|G alpha h antibody|G protein alpha subunit Gh class antibody|G[a]h antibody|Gh CLASS G ALPHA h antibody|GNAH antibody|GNAH G PROTEIN antibody|Guanine nucleotide binding protein H polypeptide antibody|H POLYPEPTIDE antibody|Protein glutamine gamma glutamyltransferase 2 antibody|Protein glutamine gamma glutamyltransferase antibody|Protein-glutamine gamma-glutamyltransferase 2 antibody|TG 2 antibody|TG(C) antibody|TG2 antibody|TGase C antibody|TGase H antibody|TGase-2 antibody|TgaseII antibody|TGC antibody|TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody|TGM 2 antibody|TGM2 antibody|TGM2_HUMAN antibody|Tissue transglutaminase antibody|Tissue type transglutaminase antibody|Transglutaminase 2 antibody|Transglutaminase 2 C polypeptide antibody|Transglutaminase C antibody|Transglutaminase H antibody|Transglutaminase-2 antibody|tTG antibody|tTGas antibody

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling