EGR1 antibody (N-Term)

Catalog No. ABIN3043997

The Rabbit Polyclonal anti-EGR1 antibody has been validated for WB. It is suitable to detect EGR1 in samples from Human. There is 1 publication available.

Quick Overview for EGR1 antibody (N-Term) (ABIN3043997)

Target: EGR1 (Early Growth Response 1 (EGR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGR1 Antibody

Binding Specificity: AA 23-43, N-Term

Purpose: Anti-Egr1 Antibody Picoband®

Sequence: FPHSPTMDNY PKLEEMMLLS N

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Egr1 Antibody (ABIN3043997). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Egr1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Das, A., Chendil, D., Dey, S., Mohiuddin, M., Mohiuddin, M., Milbrandt, J., Rangnekar, V. M., Ahmed, M. M. Ionizing radiation down-regulates p53 protein in primary Egr-1(-/-) mouse embryonic fibroblast cells causing enhanced resistance to apoptosis. J. Biol. Chem. 276: 3279-3286, 2001. 2. Joslin, J. M., Fernald, A. A., Tennant, T. R., Davis, E. M., Kogan, S. C., Anastasi, J., Crispino, J. D., Le Beau, M. M. Haploinsufficiency of EGR1, a candidate gene in the del(5q), leads to the development of myeloid disorders. Blood 110: 719-726, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-EGR1 antibody (ABIN3043997)

Lin, Fang, Cai, Huang, Zhang, Lu, Zhang, Wei: "ERK/Egr-1 signaling pathway is involved in CysLT2 receptor-mediated IL-8 production in HEK293 cells." in: European journal of cell biology, Vol. 93, Issue 7, pp. 278-88, (2014) (PubMed).

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1

Background:

Synonyms: Early growth response protein 1,EGR-1,AT225,Nerve growth factor-induced protein A,NGFI-A,Transcription factor ETR103,Transcription factor Zif268,Zinc finger protein 225,Zinc finger protein Krox-24,EGR1,KROX24, ZNF225,

Tissue Specificity: In eosinophils as well as trace amounts in neutrophils and monocytes.

Background: Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.

Sequence Similarities: Belongs to the EGR C2H2-type zinc-finger protein family.

Molecular Weight: 58 kDa

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity