HYAL1 antibody (N-Term)

Catalog No. ABIN3043999

This Rabbit Polyclonal antibody specifically detects HYAL1 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for HYAL1 antibody (N-Term) (ABIN3043999)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYAL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HYAL1 Antibody

Binding Specificity: AA 32-47, N-Term

Purpose: Anti-Hyaluronidase-1 HYAL1 Antibody Picoband®

Sequence: FTTVWNANTQ WCLERH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Hyaluronidase-1 HYAL1 Antibody (ABIN3043999). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HYAL1.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
1. Csoka, A. B., Frost, G. I., Heng, H. H. Q., Scherer, S. W., Mohapatra, G., Stern, R. The hyaluronidase gene HYAL1 maps to chromosome 3p21.2-p21.3 in human and 9F1-F2 in mouse, a conserved candidate tumor suppressor locus. Genomics 48: 63-70, 1998. Note: Erratum: Genomics 84: 227 only, 2004. 2. Martin, D. C., Atmuri, V., Hemming, R. J., Farley, J., Mort, J. S., Byers, S., Hombach-Klonisch, S., Csoka, A. B., Stern, R., Triggs-Raine, B. L. A mouse model of human mucopolysaccharidosis IX exhibits osteoarthritis. Hum. Molec. Genet. 17: 1904-1915, 2008. Note: Erratum: Hum. Molec. Genet. 17: 2919 only, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: HYAL1

Background:

Synonyms: Hyaluronidase-1,Hyal-1,3.2.1.35,Hyaluronoglucosaminidase-1,Lung carcinoma protein 1,LuCa-1,HYAL1,LUCA1,

Tissue Specificity: Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues. .

Background: Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is though to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Sequence Similarities: Belongs to the glycosyl hydrolase 56 family.

Molecular Weight: 60 kDa

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process