SLC1A3 antibody (C-Term)

Catalog No. ABIN3044001

This anti-SLC1A3 antibody is a Rabbit Polyclonal antibody detecting SLC1A3 in WB. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for SLC1A3 antibody (C-Term) (ABIN3044001)

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC1A3 Antibody

Binding Specificity: AA 519-537, C-Term

Purpose: Anti-EAAT1/SLC1A3 Antibody Picoband®

Sequence: MKKPYQLIAQ DNETEKPID

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EAAT1/SLC1A3 Antibody (ABIN3044001). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1, different from the related rat and mouse sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. de Vries, B., Mamsa, H., Stam, A. H., Wan, J., Bakker, S. L. M., Vanmolkot, K. R. J., Haan, J., Terwindt, G. M., Boon, E. M. J., Howard, B. D., Frants, R. R., Baloh, R. W., Ferrari, M. D., Jen, J. C., van den Maagdenberg, A. M. J. M. Episodic ataxia associated with EAAT1 mutation C186S affecting glutamate reuptake. Arch. Neurol. 66: 97-101, 2009. Note: Errata: Arch. Neurol. 66: 497 only, 2009, Arch. Neurol. 66: 772 only, 2009. 2. Matsugami, T. R., Tanemura, K., Mieda, M., Nakatomi, R., Yamada, K., Kondo, T., Ogawa, M., Obata, K., Watanabe, M., Hashikawa, T., Tanaka, K. Indispensability of the glutamate transporters GLAST and GLT1 to brain development. Proc. Nat. Acad. Sci. 103: 12161-12166, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SLC1A3 antibody (ABIN3044001)

Ho, Hartley, Flaherty, Rajarajan, Abdelaal, Obiorah, Barretto, Muhammad, Phatnani, Akbarian, Brennand: "Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes." in: Stem cell reports, Vol. 9, Issue 2, pp. 615-628, (2018) (PubMed).

Ding, Zhang, Liu, Zhang, Ma, Bruce, Zhang: "Tumor necrosis factor-? promotes the expression of excitatory amino-acid transporter 2 in astrocytes: Optimal concentration and incubation time." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1909-1913, (2014) (PubMed).

Target Details for SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Alternative Name: SLC1A3

Background:

Synonyms: Excitatory amino acid transporter 1,Sodium-dependent glutamate/aspartate transporter 1,GLAST-1,Solute carrier family 1 member 3,SLC1A3,EAAT1, GLAST, GLAST1,

Tissue Specificity: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Background: Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Molecular Weight: 274 kDa

UniProt: P43003

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport