Prohibitin 2 antibody (Middle Region)

Catalog No. ABIN3044022

Product Details anti-Prohibitin 2 Antibody

Target: Prohibitin 2 (PHB2)

Binding Specificity: AA 111-128, Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Prohibitin-2(PHB2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: RPNAQELPSM YQRLGLDY

Cross-Reactivity (Details): Predicted Cross Reactivity: rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Prohibitin-2(PHB2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: prohibitin 2
Protein Name: Prohibitin-2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human REA(111-128aa RPNAQELPSMYQRLGLDY), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Prohibitin 2

Target: Prohibitin 2 (PHB2)

Alternative Name: PHB2 (PHB2 Products)

Background: PHB2(Prohibitin 2), also called Repressor of Estrogen Receptor Activity(REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Synonyms: B cell receptor associated protein BAP37 antibody|B-cell associated protein antibody|B-cell receptor-associated protein BAP37 antibody|Bap 37 antibody|BAP antibody|Bap37 antibody|BCAP 37 antibody|BCAP37 antibody|D prohibitin antibody|D-prohibitin antibody|MGC117268 antibody|p22 antibody|PHB 2 antibody|PHB2 antibody|PHB2_HUMAN antibody|PNAS 141 antibody|PNAS141 antibody|Prohibitin 2 antibody|Prohibitin-2 antibody|Prohibitin2 antibody|Repressor of Estrogen Receptor Activity antibody

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling