Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP) (AA 647-666), (C-Term) antibody

Catalog No. ABIN3044029

This Rabbit Polyclonal antibody specifically detects Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein in WB, IHC, ICC, IHC (fro), FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP) (AA 647-666), (C-Term) antibody (ABIN3044029)

Target: Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 647-666, C-Term

Purpose: Anti-RIAM/APBB1IP Antibody

Sequence: EQDFMSDLMK ALQKKRGNVS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RIAM/APBB1IP Antibody (ABIN3044029). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP)

Alternative Name: APBB1IP

Background:

Synonyms: Amyloid beta A4 precursor protein-binding family B member 1-interacting protein,APBB1-interacting protein 1,Proline-rich EVH1 ligand 1,PREL-1,Proline-rich protein 73,Rap1-GTP-interacting adapter molecule,RIAM,Retinoic acid-responsive proline-rich protein 1,RARP-1,APBB1IP,PREL1, RARP1, RIAM,

Tissue Specificity: Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. .

Background: APBB1IP (APBB1-Interacting Protein), also called RIAM or RARP1, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the RIAM gene to chromosome 10p12.1. Using promoter-< a href="https://www.bosterbio.com/cells/reporter-cell-lines" style="color:#ea8d28">reporter gene assays, Inagaki et al. (2003) found that RARP1 suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RARP1 suppressed AP1 transactivation. Lafuente et al. (2004) found that RIAM interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.

Sequence Similarities: Belongs to the MRL family.

Molecular Weight: 110-120 kDa

UniProt: Q7Z5R6