CUEDC2 antibody (C-Term)

Catalog No. ABIN3044031

The Rabbit Polyclonal anti-CUEDC2 antibody (ABIN3044031) specifically detects CUEDC2 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CUEDC2 antibody (C-Term) (ABIN3044031)

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CUEDC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CUEDC2 Antibody

Binding Specificity: C-Term

Purpose: Anti-CUEDC2 Antibody

Sequence: EAEEMKATYI NLKPARKYRF H

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse sequence,and different from the related rat sequence by one amino acid.

Characteristics: Anti-CUEDC2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, RatWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CUEDC2

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Alternative Name: CUEDC2

Background:

Background: CUEDC2 (Cue Domain-Containing Protein 2) is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor (PR, or PGR) and estrogen receptor (ER)-alpha. Hartz (2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the CUEDC2 sequence with the genomic sequence (GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al. (2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.

Gene Full Name: CUE domain containing 2

Sequence Similarities: Belongs to the CUEDC2 family.

Molecular Weight: 32 kDa, 19 kDa

Gene ID: 79004

UniProt: Q9H467