DDX5 antibody (C-Term)

Catalog No. ABIN3044033

Product Details anti-DDX5 Antibody

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Binding Specificity: AA 566-580, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX5(DDX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: TYQNGYDSTQ QYGSN

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX5(DDX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: DEAD(Asp-Glu-Ala-Asp) box helicase 5
Protein Name: Probable ATP-dependent RNA helicase DDX5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5(566-580aa TYQNGYDSTQQYGSN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for DDX5 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5 (DDX5 Products)

Synonyms: G17P1 antibody, HLR1 antibody, HUMP68 antibody, p68 antibody, 2600009A06Rik antibody, Hlr1 antibody, wu:fa56a07 antibody, wu:fb11e01 antibody, wu:fb16c10 antibody, wu:fb53b05 antibody, ddx5 antibody, DEAD-box helicase 5 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 5 antibody, DEAD-box helicase 5 L homeolog antibody, DDX5 antibody, Ddx5 antibody, ddx5 antibody, ddx5.L antibody

Background: DDX5(DEAD/H BOX 5), also known as HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. The p68 protein is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen(Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al.(1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al.(2006) found that p68, p72(DDX17), and the noncoding RNA SRA(SRA1) associated with MYOD(MYOD1) in MYOD-transfected HeLa cells.

Synonyms: ATP dependent RNA helicase DDX5 antibody|DDX 5 antibody|Ddx5 antibody|DDX5_HUMAN antibody|DEAD(Asp Glu Ala Asp) box helicase 5 antibody|DEAD(Asp Glu Ala Asp) box polypeptide 5 antibody|DEAD box 5 antibody|DEAD box protein 5 antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5(RNA helicase, 68kD) antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 5(RNA helicase 68 kDa) antibody|DEAD/H box-5(RNA helicase 68kD) antibody|DKFZp686J01190 antibody|G17P1 antibody|HELR antibody|HLR 1 antibody|HLR1 antibody|HUMP68 antibody|P68 antibody|p68 RNA helicase antibody|Probable ATP dependent RNA helicase DDX5 antibody|Probable ATP-dependent RNA helicase DDX5 antibody|RNA helicase 68kD antibody|RNA helicase p68 antibody|RNA-dependent ATPase antibody

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus