PDPK1 antibody (C-Term)

Catalog No. ABIN3044043

This anti-PDPK1 antibody is a Rabbit Polyclonal antibody detecting PDPK1 in WB and IHC. Suitable for Human and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PDPK1 antibody (C-Term) (ABIN3044043)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 408-421, C-Term

Purpose: Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®

Sequence: WKHYNTNHEA DDWC

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody (ABIN3044043). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-PDPK1 antibody (ABIN3044043)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Xu, Ding, Fang, Zheng, Gao, Lu, Zhang: "Silencing of HIF-1alpha suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis." in: Cancer gene therapy, Vol. 17, Issue 3, pp. 212-22, (2010) (PubMed).

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background:

Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial,2.7.11.2,Pyruvate dehydrogenase kinase isoform 1,PDH kinase 1,PDK1,PDHK1,

Tissue Specificity: Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. .

Background: PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Sequence Similarities: Belongs to the PDK/BCKDK protein kinase family.

Molecular Weight: 85-91 kDa

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals