RGS3 antibody (C-Term)

Catalog No. ABIN3044065

This Rabbit Polyclonal antibody specifically detects RGS3 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RGS3 antibody (C-Term) (ABIN3044065)

Target: RGS3 (Regulator of G-Protein Signaling 3 (RGS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RGS3 Antibody

Binding Specificity: AA 909-925, C-Term

Purpose: Anti-RGS3 Antibody Picoband®

Sequence: DSYTREHTKE NLQSITR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RGS3 Antibody (ABIN3044065). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse RGS3, identical to the related rat sequence, and different from the related human sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Chatterjee, T. K., Eapen, A., Kanis, A. B., Fisher, R. A. Genomic organization, 5-prime-flanking region, and chromosomal localization of the human RGS3 gene. Genomics 45: 429-433, 1997. 2. Druey, K. M., Blumer, K. J., Kang, V. H., Kehrl, J. H. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature 379: 742-746, 1996. 3. Lu, Q., Sun, E. E., Klein, R. S., Flanagan, J. G. Ephrin-B reverse signaling is mediated by a novel PDZ-RGS protein and selectively inhibits G protein-coupled chemoattraction. Cell 105: 69-79, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RGS3

Target: RGS3 (Regulator of G-Protein Signaling 3 (RGS3))

Alternative Name: Rgs3

Background:

Synonyms: Regulator of G-protein signaling 3,RGS3,C2PA,Rgs3,

Tissue Specificity: Detected in embryos from E8.5-16.5 in cortical ventricular zone, dorsal root ganglia and cerebellar primordia. Isoform 3 is detected in testis and in spermatocytes from newborn mice. Levels increase and reach a maximum after 21 days, after this they decrease again. Long isoforms are widely expressed. .

Background: RGS3 (Regulator of G Protein Signaling 3), is a protein that in humans is encoded by the RGS3 gene. Chatterjee et al. (1997) studied RGS3, the largest member of the RGS family then identified. Mouse Pdz-Rgs3 can mediate signaling from the ephrin-B cytoplasmic tail. By radiation hybrid mapping, Chatterjee et al. (1997) assigned the RGS3 gene to chromosome 9q31-q33. Lu et al. (2001) showed that Sdf1, a chemokine with a G protein-coupled receptor, and Bdnf are chemoattractants for cerebellar granule cells, and that Sdf1 chemoattraction is selectively inhibited by soluble ephrin-B receptor. This inhibition could be blocked by a truncated Pdz-Rgs3 protein lacking the RGS domain.

Sequence Similarities: Contains 1 PDZ (DHR) domain.

Molecular Weight: 93 kDa

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling