FABP5 antibody (N-Term)

Catalog No. ABIN3044084

The Rabbit Polyclonal anti-FABP5 antibody is suitable to detect FABP5 in samples from Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for FABP5 antibody (N-Term) (ABIN3044084)

Target: FABP5 (Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FABP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FABP5 Antibody

Binding Specificity: AA 10-23, N-Term

Purpose: Anti-Fatty Acid Binding Protein 5/FABP5 Antibody

Sequence: KWRLMESHGF EEYM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Fatty Acid Binding Protein 5/FABP5 Antibody (ABIN3044084). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Fatty Acid Binding Protein 5, different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FABP5

Target: FABP5 (Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5))

Alternative Name: FABP5

Background:

Synonyms: Fatty acid-binding protein, epidermal;Epidermal-type fatty acid-binding protein;E-FABP;Fatty acid-binding protein 5;Keratinocyte lipid-binding protein;Psoriasis-associated fatty acid-binding protein homolog;PA-FABP;Fabp5;Fabpe, Klbp, Mal1;

Tissue Specificity: Most abundant in keratinocytes and also in stratified epithelia of epidermis and tongue. Relatively high levels found in adipose and mammary tissues and small amounts found in heart, brain, liver, spleen, muscle and lung.

Background: FABP5 (fatty acid binding protein 5 (psoriasis-associated)) is a protein that in humans is encoded by the FABP5 gene, also known as PAFABP, EFABP, E-FABP, KFABP, PA-FABP, Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog. It is a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. It is though that FABPs roles include fatty acid uptake, transport, and metabolism. Kaczocha et al. (2009) identified FABP5 and FABP7 as cytosolic proteins that transport AEA from the plasma membrane to subcellular fatty acid amide hydrolase, where it is hydrolyzed and inactivated. FABP3 did not show this specific transport function.

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 15 kDa

UniProt: Q05816