FER antibody (Middle Region)

Catalog No. ABIN3044101

This Rabbit Polyclonal antibody specifically detects FER in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for FER antibody (Middle Region) (ABIN3044101)

Target: FER (FER Tyrosine Kinase (FER))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FER antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FER Antibody

Binding Specificity: Middle Region

Purpose: Anti-Tyrosine-protein kinase Fer FER Antibody

Sequence: FSNIPQLIDH HYTTKQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by two amino acids.

Characteristics: Anti-Tyrosine-protein kinase Fer FER Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FER, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, RatWestern blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FER

Target: FER (FER Tyrosine Kinase (FER))

Alternative Name: FER

Background:

Background: FER (FPS/FES-Related tyrosine kinase) also known as TYK3, is an enzyme that in humans is encoded by the FER gene. Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. By in situ hybridization, Morris et al. (1990) concluded that the FER gene is located at 5q21-q22. Treatment of cells with JMP resulted in the release of FER from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1. In Fer mutant mice, leukocyte emigration was exaggerated in response to LPS without altering vascular permeability, suggesting that FER has a role in regulating innate immunity.

Gene Full Name: FER tyrosine kinase

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

Molecular Weight: 95 kDa

Gene ID: 2241

UniProt: P16591