GATA2 antibody (N-Term)

Catalog No. ABIN3044102

The Rabbit Polyclonal anti-GATA2 antibody has been validated for WB. It is suitable to detect GATA2 in samples from Human, Mouse and Rat.

Quick Overview for GATA2 antibody (N-Term) (ABIN3044102)

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GATA2 Antibody

Binding Specificity: AA 46-60, N-Term

Purpose: Anti-GATA2 Antibody Picoband®

Sequence: DVFFNHLDSQ GNPYY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GATA2 Antibody (ABIN3044102). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GATA2, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Ciciotte, S. L., Tsai, F.-Y., Peters, L. L.Gata2 maps to mouse chromosome 6.Mammalian Genome 8: 69-70, 1997. 2. Dorfman, D. M., Wilson, D. B., Bruns, G. A., Orkin, S. H.Human transcription factor GATA-2: evidence for regulation of preproendothelin-1 gene expression in endothelial cells.J. Biol. Chem. 267: 1279-1285, 1992. 3. Wieser, R., Volz, A., Vinatzer, U., Gardiner, K., Jager, U., Mitterbauer, M., Ziegler, A., Fonatsch, C.Transcription factor GATA-2 gene is located near 3q21 breakpoints in myeloid leukemia.Biochem. Biophys. Res. Commun. 273: 239-245, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA2

Background:

Synonyms: Endothelial transcription factor GATA-2,GATA-binding protein 2,GATA2,

Tissue Specificity: Endothelial cells.

Background: GATA2 (GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al. (1997)mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al. (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al. (2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

Sequence Similarities: Contains 2 GATA-type zinc fingers.

Molecular Weight: 95 kDa

UniProt: P23769

Pathways: Stem Cell Maintenance