KLF5 antibody (Middle Region)

Catalog No. ABIN3044106

Product Details anti-KLF5 Antibody

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Binding Specificity: AA 109-126, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Krueppel-like factor 5(KLF5) detection. Tested with WB in Human,Mouse,Rat.

Sequence: HKKYRRDSAS VVDQFFTD

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Krueppel-like factor 5(KLF5) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: Kruppel-like factor 5(intestinal)
Protein Name: Krueppel-like factor 5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human KLF5(109-126aa HKKYRRDSASVVDQFFTD), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for KLF5 is approximately 1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for KLF5

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Alternative Name: KLF5 (KLF5 Products)

Synonyms: KLF5 antibody, klf5 antibody, MGC115081 antibody, BTEB2 antibody, Bteb2 antibody, IKLF antibody, bteb2 antibody, 4930520J07Rik antibody, CKLF antibody, Kruppel like factor 5 antibody, Kruppel-like factor 5 (intestinal) S homeolog antibody, Kruppel-like factor 5 antibody, KLF5 antibody, klf5.S antibody, Klf5 antibody

Background: KLF5(Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al.(2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al.(1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59 % and 64 % identical to those of Sp1(189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al.(1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

Synonyms: Basic transcription element binding protein 2 antibody|Basic transcription element-binding protein 2 antibody|BTE binding protein 2 antibody|BTE-binding protein 2 antibody|BTEB 2 antibody|BTEB2 antibody|CKLF antibody|Colon krueppel like factor antibody|Colon krueppel-like factor antibody|GC box binding protein 2 antibody|GC-box-binding protein 2 antibody|IKLF antibody|Intestinal enriched krueppel like factor antibody|Intestinal-enriched krueppel-like factor antibody|KLF 5 antibody|Klf5 antibody|KLF5_HUMAN antibody|Krueppel like factor 5 antibody|Krueppel-like factor 5 antibody|Kruppel like factor 5 intestinal antibody|Transcription factor BTEB 2 antibody|Transcription factor BTEB2 antibody

UniProt: Q13887