CACYBP antibody (Middle Region)

Catalog No. ABIN3044149

This Rabbit Polyclonal antibody specifically detects CACYBP in WB, IF, FACS, ICC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CACYBP antibody (Middle Region) (ABIN3044149)

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACYBP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CACYBP Antibody

Binding Specificity: Middle Region

Purpose: Anti-SIAH Interacting Protein/CACYBP Antibody

Sequence: NTRWDYLTQV EKECKE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-SIAH Interacting Protein/CACYBP Antibody. Tested in IP, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human Immunoprecipitation, 0.5-2 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CACYBP

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Alternative Name: CACYBP

Background:

Background: CACYBP (Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

Gene Full Name: calcyclin binding protein

Sequence Similarities: Contains 1 CS domain.

Molecular Weight: 27 kDa

Gene ID: 27101

UniProt: Q9HB71

Pathways: Response to Growth Hormone Stimulus