DCI antibody (C-Term)

Catalog No. ABIN3044153

The Rabbit Polyclonal anti-DCI antibody is suitable to detect DCI in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for DCI antibody (C-Term) (ABIN3044153)

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DCI Antibody

Binding Specificity: C-Term

Purpose: Anti-DCI/ECI1 Antibody

Sequence: ADVQNFVSFI SKDSIQKSL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids,from the related rat sequence by three amino acids.

Characteristics: Anti-DCI/ECI1 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DCI

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Alternative Name: ECI1

Background:

Background: ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

Gene Full Name: enoyl-CoA delta isomerase 1

Molecular Weight: 28 kDa

Gene ID: 1632

UniProt: P42126