GLI3 antibody (N-Term)

Catalog No. ABIN3044156

The Rabbit Polyclonal anti-GLI3 antibody has been validated for WB. It is suitable to detect GLI3 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for GLI3 antibody (N-Term) (ABIN3044156)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLI3 Antibody

Binding Specificity: AA 40-57, N-Term

Purpose: Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

Sequence: SNEDESPGQT YHRERRNA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcriptional activator GLI3 Gli3 Antibody (ABIN3044156). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early stages of limb development. (Letter) Nature 436: 277-281, 2005. 2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Molec. Genet. 11: 1129-1135, 2002. 3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-GLI3 antibody (ABIN3044156)

Kumari, Chaurasia, Kumar, Dash: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background:

Synonyms: Transcriptional activator GLI3,GLI3 form of 190 kDa,GLI3-190,GLI3 full length protein,GLI3FL,Transcriptional repressor GLI3R,GLI3 C-terminally truncated form,GLI3 form of 83 kDa,GLI3-83,GLI3,

Tissue Specificity: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.

Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

Molecular Weight: 33 kDa

UniProt: P10071

Pathways: Hedgehog Signaling