LASP1 antibody (N-Term)

Catalog No. ABIN3044177

This Rabbit Polyclonal antibody specifically detects LASP1 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LASP1 antibody (N-Term) (ABIN3044177)

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LASP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-LASP1 Antibody

Binding Specificity: N-Term

Purpose: Anti-LIM and SH3 domain protein 1 LASP1 Antibody

Sequence: EKVNCLDKFW HKACF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat sequence,different from the related mouse sequence by one amino acid.

Characteristics: Anti-LIM and SH3 domain protein 1 LASP1 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1, identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, RatWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LASP1

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Alternative Name: LASP1

Background:

Background: LASP1 (LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al. (1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8 % of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

Gene Full Name: LIM and SH3 protein 1

Sequence Similarities: Contains 1 LIM zinc-binding domain.

Molecular Weight: 11 kDa

Gene ID: 3927

UniProt: Q14847