HOXA4 antibody (N-Term)

Catalog No. ABIN3044213

Product Details anti-HOXA4 Antibody

Target: HOXA4 (Homeobox A4 (HOXA4))

Binding Specificity: AA 9-26, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Homeobox protein Hox-A4(HOXA4) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: NSNYIEPKFP PFEEYAQH

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Homeobox protein Hox-A4(HOXA4) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: homeobox A4
Protein Name: Homeobox protein Hox-A4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HOXA4(9-26aa NSNYIEPKFPPFEEYAQH), different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-HOXA4 antibody (ABIN3044213)

Li, Chen, Su, Hu, Li, Elkahloun, Zuo, Gurbuxani, Arnovitz, Weng, Wang, Li, Huang, Neilly, Wang, Jiang, Liu, Jin, Chen: "PBX3 and MEIS1 Cooperate in Hematopoietic Cells to Drive Acute Myeloid Leukemias Characterized by a Core Transcriptome of the MLL-Rearranged Disease." in: Cancer research, Vol. 76, Issue 3, pp. 619-29, (2016) (PubMed).

Target Details for HOXA4

Target: HOXA4 (Homeobox A4 (HOXA4))

Alternative Name: HOXA4 (HOXA4 Products)

Background: HOXA4(HOMEOBOX A4), also known as HOX1D or HOMOLOG OF, is a protein that in humans is encoded by the HOXA4 gene, which is also part of the A cluster on chromosome 7. HOXA4 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.

Synonyms: Dfd like protein antibody|Homeo box A4 antibody|Homeobox A4 antibody|Homeobox protein Hox-1.4 antibody|Homeobox protein Hox-1D antibody|Homeobox protein Hox-A4 antibody|Hox 1.4 like protein antibody|hox-1.4 antibody|hox-1d antibody|HOX1 antibody|HOX1D antibody|HOXA4 antibody|HXA4_HUMAN antibody

UniProt: Q00056