XRCC1 antibody (N-Term)

Catalog No. ABIN3044234

The Rabbit Polyclonal anti-XRCC1 antibody has been validated for WB, IHC and ICC. It is suitable to detect XRCC1 in samples from Human, Mouse and Rat.

Quick Overview for XRCC1 antibody (N-Term) (ABIN3044234)

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-XRCC1 Antibody

Binding Specificity: AA 15-34, N-Term

Purpose: Anti-XRCC1 Antibody Picoband®

Sequence: QDSTHCAENL LKADTYRKWR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-XRCC1 Antibody (ABIN3044234). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Bhattacharyya, N., Banerjee, S. A novel role of XRCC1 in the functions of a DNA polymerase beta variant. Biochemistry 40: 9005-9013, 2001. 2. Brookman, K. W., Tebbs, R. S., Allen, S. A., Tucker, J. D., Swiger, R. R., Lamerdin, J. E., Carrano, A. V., Thompson, L. H. Isolation and characterization of mouse Xrcc1, a DNA repair gene affecting ligation. Genomics 22: 180-188, 1994.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for XRCC1

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Alternative Name: XRCC1

Background:

Synonyms: DNA repair protein XRCC1,X-ray repair cross-complementing protein 1,XRCC1,

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Sequence Similarities: Contains 2 BRCT domains.

Molecular Weight: 90 kDa

UniProt: P18887

Pathways: DNA Damage Repair