MCM5 antibody (C-Term)

Catalog No. ABIN3044244

This anti-MCM5 antibody is a Rabbit Polyclonal antibody detecting MCM5 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for MCM5 antibody (C-Term) (ABIN3044244)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MCM5 Antibody

Binding Specificity: AA 720-734, C-Term

Purpose: Anti-MCM5 Antibody Picoband®

Sequence: EIQHRMQRKV LYRLK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCM5 Antibody (ABIN3044244). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 2. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 3. Paul R, Hu B, Musahl C, Hameister H, Knippers R (Sep 1996). "Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5". Cytogenet Cell Genet 73 (4): 317-21.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background:

Synonyms: DNA replication licensing factor MCM5,3.6.4.12,CDC46 homolog,P1-CDC46,MCM5,CDC46,

Tissue Specificity: Heart, liver, duodenum, blood vessels and kidney.

Background: MCM5 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Sequence Similarities: Belongs to the MCM family.

Molecular Weight: 82 kDa

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA