ALDH1A1 antibody (Middle Region)

Catalog No. ABIN3044255

There is 1 publication for this product available. The Rabbit Polyclonal anti-ALDH1A1 antibody is suitable to detect ALDH1A1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, IF and ICC.

Quick Overview for ALDH1A1 antibody (Middle Region) (ABIN3044255)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ALDH1A1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

Sequence: DLDNAVEFAH HGVFYHQGQC C

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Characteristics: Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-ALDH1A1 antibody (ABIN3044255)

Shao, Fan, Ma, Wu: "Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 4991-4998, (2017) (PubMed).

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Background:

Background: ALDH1A1 (Aldehyde dehydrogenase 1 family, member A1), also called ALDH1, RALDH1 or ALDH, LIVER CYTOSOLIC, is an enzyme that in humans is encoded by the ALDH1A1 gene. And this protein belongs to the aldehyde dehydrogenases family of proteins. The ALDH1A1 gene is mapped on 9q21.13. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea.ALDH1A1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. The ALDH1 gene is about 53 kb long and is divided into 13 exons. Retinaldehyde is generated by ADH1 from retinol, and its concentration is determined in large part by its subsequent catabolism by RALDH1 to retinoic acid.

Gene Full Name: aldehyde dehydrogenase 1 family member A1

Sequence Similarities: Belongs to the aldehyde dehydrogenase family.

Molecular Weight: 65 kDa

Gene ID: 216

UniProt: P00352

Pathways: Dopaminergic Neurogenesis