PAK6 antibody (N-Term)

Catalog No. ABIN3044285

The Rabbit Polyclonal anti-PAK6 antibody is suitable to detect PAK6 in samples from Human, Mouse and Rat. It has been validated for WB, IF, ICC and FACS.

Quick Overview for PAK6 antibody (N-Term) (ABIN3044285)

Target: PAK6 (P21-Activated Kinase 6 (PAK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PAK6 Antibody

Binding Specificity: N-Term

Purpose: Anti-PAK6 Antibody

Sequence: QNFQHRVHTS FDPKEGK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-PAK6 Antibody. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PAK6

Target: PAK6 (P21-Activated Kinase 6 (PAK6))

Alternative Name: PAK6

Background:

Background: Serine/threonine-protein kinase PAK 6 (p21 Protein-activated Kinase 6) is an enzyme that in humans is encoded by the PAK6 gene. The PAK6 gene is mapped to chromosome 15q15. based on an alignment of the PAK6 sequence with the genomic sequence. This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.

Gene Full Name: BUB1B-PAK6 readthrough

Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Molecular Weight: 70-75 kDa

Gene ID: 106821730

UniProt: Q9NQU5