CD40 Ligand antibody (N-Term)

Catalog No. ABIN3044319

Cited in 1 publication. The Rabbit Polyclonal anti-CD40 Ligand antibody (ABIN3044319) specifically detects CD40 Ligand in WB and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for CD40 Ligand antibody (N-Term) (ABIN3044319)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD40 Ligand Antibody

Binding Specificity: N-Term

Purpose: Anti-CD40L/CD40LG Antibody

Sequence: HRRLDKIEDE RNLHEDFVF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by three amino acids,rat sequence by four amino acids.

Characteristics: Anti-CD40L/CD40LG Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD40L, different from the related mouse sequence by three amino acids, rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD40 Ligand antibody (ABIN3044319)

Yang, Fang, Liu, Zhang, Shi, Ji, Lin, Lin: "Protective effects of chinese traditional medicine buyang huanwu decoction on myocardial injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 930324, (2012) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG

Background:

Background: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells. CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Gene Full Name: CD40 ligand

Sequence Similarities: Belongs to the tumor necrosis factor family.

Molecular Weight: 29 kDa

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints