Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) antibody

Catalog No. ABIN3044322

The Rabbit Polyclonal anti-Deoxyuridine Triphosphatase antibody (ABIN3044322) specifically detects Deoxyuridine Triphosphatase in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) antibody (ABIN3044322)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 212-229, C-Term

Purpose: Anti-DUT Antibody

Sequence: KKGDRIAQLI CERIFYPE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DUT Antibody (ABIN3044322). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background:

Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial,dUTPase,3.6.1.23,dUTP pyrophosphatase,DUT,

Tissue Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). .

Background: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E.coli dUTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Sequence Similarities: Belongs to the dUTPase family.

Molecular Weight: 27 kDa

UniProt: P33316