SOCS1 antibody (C-Term)

Catalog No. ABIN3044331

This anti-SOCS1 antibody is a Rabbit Polyclonal antibody detecting SOCS1 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for SOCS1 antibody (C-Term) (ABIN3044331)

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SOCS1 Antibody

Binding Specificity: AA 197-211, C-Term

Purpose: Anti-SOCS1 Antibody Picoband®

Sequence: NPVLRDYLSS FPFQI

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SOCS1 Antibody (ABIN3044331). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Ohya K, Kajigaya S, Yamashita Y, Miyazato A, Hatake K, Miura Y, Ikeda U, Shimada K, Ozawa K, Mano H.SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase. J Biol Chem. 1997 Oct 24, 272(43):27178-82. 2. Kramer JA, Adams MD, Singh GB, Doggett NA, Krawetz SA.A matrix associated region localizes the human SOCS-1 gene to chromosome 16p13.13. Somat Cell Mol Genet. 1998 Mar, 24(2):131-3. 3. Nagai H, Naka T, Terada Y, Komazaki T, Yabe A, Jin E, Kawanami O, Kishimoto T, Konishi N, Nakamura M, Kobayashi Y, Emi M.Hypermethylation associated with inactivation of the SOCS-1 gene, a JAK/STAT inhibitor, in human hepatoblastomas. J Hum Genet. 2003, 48(2):65-9. 4. Yoshida T, Ogata H, Kamio M, Joo A, Shiraishi H, Tokunaga Y, Sata M, Nagai H, Yoshimura A.SOCS1 is a suppressor of liver fibrosis and hepatitis-induced carcinogenesis. J Exp Med. 2004 Jun 21, 199(12):1701-7.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-SOCS1 antibody (ABIN3044331)

Yang, Tian, Zhang, Xu, Yang, Yu, Li, Li, Li, Li: "Role of the JAK2/STAT3 signaling pathway in the pathogenesis of type 2 diabetes mellitus with macrovascular complications." in: Oncotarget, Vol. 8, Issue 57, pp. 96958-96969, (2017) (PubMed).

Wan, Ma, Mei, Shan: "The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 150, (2010) (PubMed).

Target Details for SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Alternative Name: SOCS1

Background:

Synonyms: Suppressor of cytokine signaling 1,SOCS-1,JAK-binding protein,JAB,STAT-induced STAT inhibitor 1,SSI-1,Tec-interacting protein 3,TIP-3,SOCS1,SSI1, TIP3,

Tissue Specificity: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Background: Suppressor of cytokine signaling 1 (SOCS1), also known as Jak-binding protein (JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211-amino acid protein has a molecular mass of about 23.5 kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13. SOCS1 regulates the JAK/STAT signal-transduction pathway. Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis.

Sequence Similarities: Contains 1 SH2 domain.

Molecular Weight: 62-65 kDa

UniProt: O15524

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, TLR Signaling, Response to Growth Hormone Stimulus