Cullin 4B antibody (C-Term)

Catalog No. ABIN3044348

The Rabbit Polyclonal anti-Cullin 4B antibody has been validated for WB and IHC. It is suitable to detect Cullin 4B in samples from Human, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for Cullin 4B antibody (C-Term) (ABIN3044348)

Target: Cullin 4B (CUL4B)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cullin 4B Antibody

Binding Specificity: AA 895-913, C-Term

Purpose: Anti-Cullin 4B/CUL4B Antibody Picoband®

Sequence: DRDYMERDKE NPNQYNYIA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cullin 4B/CUL4B Antibody (ABIN3044348). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Zebrafish
Western blot, 0.1-0.5 μg/mL, Human, Rat, Zebrafish, Mouse
1. Zou, Y., Liu, Q., Chen, B., Zhang, X., Guo, C., Zhou, H., Li, J., Gao, G., Guo, Y., Yan, C., Wei, J., Shao, C., Gong, Y. : Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation. Am. J. Hum. Genet. 80: 561-566, 2007. 2. Tarpey, P. S., Raymond, F. L., O'Meara, S., Edkins, S., Teague, J., Butler, A., Dicks, E., Stevens, C., Tofts, C., Avis, T., Barthorpe, S., Buck, G. : {and 41 others}: Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor. Am. J. Hum. Genet. 80: 345-352, 2007. 3. Ohtake, F., Baba, A., Takada, I., Okada, M., Iwasaki, K., Miki, H., Takahashi, S., Kouzmenko, A., Nohara, K., Chiba, T., Fujii-Kuriyama, Y., Kato, S. : Dioxin receptor is a ligand-dependent E3 ubiquitin ligase. Nature 446: 562-566, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cullin 4B

Target: Cullin 4B (CUL4B)

Alternative Name: CUL4B

Background:

Synonyms: Cullin-4B,CUL-4B,CUL4B,KIAA0695,

Tissue Specificity: Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Background: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

Sequence Similarities: Belongs to the cullin family.

Molecular Weight: 104 kDa

UniProt: Q13620