Annexin A2 antibody (Middle Region)

Catalog No. ABIN3044380

This Rabbit Polyclonal antibody specifically detects Annexin A2 in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for Annexin A2 antibody (Middle Region) (ABIN3044380)

Target: Annexin A2 (ANXA2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Annexin A2 Antibody

Binding Specificity: AA 121-141, Middle Region

Purpose: Anti-Annexin A2/ANXA2 Antibody

Sequence: RAEDGSVIDY ELIDQDARDL Y

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Annexin A2/ANXA2 Antibody (ABIN3044380). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2, different from the rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Annexin A2 antibody (ABIN3044380)

Zhu, Zou, Jia, Zhou, Hu, Peng, Chen, Jin: "Analysis of cellular proteome alterations in porcine alveolar macrophage cells infected with 2009 (H1N1) and classical swine H1N1 influenza viruses." in: Journal of proteomics, Vol. 75, Issue 6, pp. 1732-41, (2012) (PubMed).

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: ANXA2

Background:

Synonyms: Annexin A2,Annexin II,Annexin-2,Calpactin I heavy chain,Calpactin-1 heavy chain,Chromobindin-8,Lipocortin II,Placental anticoagulant protein IV,PAP-IV,Protein I,p36,ANXA2,ANX2, ANX2L4, CAL1H, LPC2D,

Background: Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. The ANXA2 gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. Richard et al. (1994) presented an integration of the physical, expression, and genetic maps of human chromosome 15. They placed the ANXA2 gene in their region IV, i.e., 15q21-q22, thus confirming the previous localization.

Sequence Similarities: Belongs to the annexin family.

Molecular Weight: 36 kDa

UniProt: P07355

Pathways: S100 Proteins