IBSP antibody (N-Term)

Catalog No. ABIN3044423

The Rabbit Polyclonal anti-IBSP antibody has been validated for WB and IHC. It is suitable to detect IBSP in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for IBSP antibody (N-Term) (ABIN3044423)

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IBSP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IBSP Antibody

Binding Specificity: AA 17-34, N-Term

Purpose: Anti-Bone Sialoprotein/IBSP Antibody Picoband®

Sequence: FSMKNLHRRV KIEDSEEN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Bone Sialoprotein/IBSP Antibody (ABIN3044423). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein, different from the related rat and mouse sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Duncan EL, et al. Genome-wide association study using extreme truncate selection identifies novel genes affecting bone mineral absorbance and fracture risk. PLoS Genet, 2011 Apr. 2. Gordon JA, et al. Bone sialoprotein stimulates focal adhesion-related signaling pathways: role in migration and survival of breast and prostate cancer cells. J Cell Biochem, 2009 Aug 15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IBSP antibody (ABIN3044423)

Zhou, Deng, Liu, Li: "Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 485-491, (2016) (PubMed).

Cheng, Wang, Zhang, Shi: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).

Target Details for IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Alternative Name: IBSP

Background:

Synonyms: Bone sialoprotein 2,Bone sialoprotein II,BSP II,Cell-binding sialoprotein,Integrin-binding sialoprotein,IBSP,BNSP,

Tissue Specificity: Ubiquitous.

Background: BSP is also known as IBSP (integrin-binding sialoprotein). The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Sequence Similarities: Contains 1 CARD domain.

Molecular Weight: 37 kDa

UniProt: P21815