POU2AF1 antibody (C-Term)

Catalog No. ABIN3044424

The Rabbit Polyclonal anti-POU2AF1 antibody (ABIN3044424) specifically detects POU2AF1 in WB and IHC. The antibody is reactive with Rat and Mouse samples.

Quick Overview for POU2AF1 antibody (C-Term) (ABIN3044424)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-POU2AF1 Antibody

Binding Specificity: C-Term

Purpose: Anti-BOB1/POU2AF1 Antibody

Sequence: EEEESNTYEL NHTLSVEGF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by one amino acid,and from human sequence by four amino acids.

Characteristics: Anti-BOB1/POU2AF1 Antibody. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1

Background:

Background: POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by ly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

Gene Full Name: POU domain, class 2, associating factor 1

Molecular Weight: 37 kDa

Gene ID: 18985

UniProt: Q64693