CAMKK1 antibody (N-Term)

Catalog No. ABIN3044431

Product Details anti-CAMKK1 Antibody

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Binding Specificity: AA 10-31, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Calcium/calmodulin-dependent protein kinase kinase 1(CAMKK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: QDPRAELVDR VAAINVAHLE EA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Calcium/calmodulin-dependent protein kinase kinase 1(CAMKK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha
Protein Name: Calcium/calmodulin-dependent protein kinase kinase 1(CaM-KK 1/CaM-kinase kinase 1/CaMKK 1)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK(10-31aa QDPRAELVDRVAAINVAHLEEA), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CAMKK1

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Alternative Name: CAMKK1 (CAMKK1 Products)

Synonyms: cam-kk antibody, cam-kk1 antibody, camkka antibody, zgc:113440 antibody, CAMKK1 antibody, CaM-KK antibody, CAMKKA antibody, AI846603 antibody, CaMKKalpha antibody, Camkk antibody, calcium/calmodulin-dependent protein kinase kinase 1 S homeolog antibody, calcium/calmodulin dependent protein kinase kinase 1 antibody, calcium/calmodulin-dependent protein kinase kinase 1, alpha a antibody, calcium/calmodulin-dependent protein kinase kinase 1 antibody, calcium/calmodulin-dependent protein kinase kinase 1, alpha antibody, camkk1.S antibody, CAMKK1 antibody, camkk1a antibody, camkk1 antibody, Camkk1 antibody

Background: CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca(2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Synonyms: Ca2+/calmodulin-dependent protein kinase kinase alpha antibody|Calcium/calmodulin dependent protein kinase kinase antibody|Calcium/calmodulin dependent protein kinase kinase 1 alpha antibody|Calcium/calmodulin dependent protein kinase kinase 1 antibody|Calcium/calmodulin dependent protein kinase kinase alpha antibody|Calcium/calmodulin-dependent protein kinase kinase 1 antibody|Calcium/calmodulin-dependent protein kinase kinase alpha antibody|CaM kinase kinase alpha antibody|CaM-kinase IV kinase antibody|CaM-kinase kinase 1 antibody|CaM-kinase kinase alpha antibody|CaM-KK 1 antibody|CaM-KK alpha antibody|CaMKK 1 antibody|CaMKK alpha antibody|CAMKK alpha protein antibody|CaMKK antibody|CAMKK1 antibody|CAMKKA antibody|DKFZp761M0423 antibody|KKCC1_HUMAN antibody|MGC34095 antibody|OTTMUSP00000006419 antibody

Pathways: AMPK Signaling