E2F1 antibody (Middle Region)

Catalog No. ABIN3044440

This Rabbit Polyclonal antibody specifically detects E2F1 in WB. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for E2F1 antibody (Middle Region) (ABIN3044440)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 246-260, Middle Region

Purpose: Anti-Transcription factor E2F1 E2F1 Antibody Picoband®

Sequence: VTCQDLRSIA DPAEQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcription factor E2F1 E2F1 Antibody (ABIN3044440). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human , Mouse, Rat
1. Neuman E, Sellers WR, McNeil JA, Lawrence JB, Kaelin WG Jr (Dec 1996). "Structure and partial genomic sequence of the human E2F1 gene". Gene 173 (2): 163-9. 2. Morris, E. J., Ji, J.-Y., Yang, F., DiStefano, L., Herr, A., Moon, N.-S., Kwon, E.-J., Haigis, K. M., Naar, A. M., Dyson, N. J. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 455: 552-556, 2008. 3. Neuman, E., Sellers, W. R., McNeil, J. A., Lawrence, J. B., Kaelin, W. G., Jr. Structure and partial genomic sequence of the human E2F1 gene. Gene 173: 163-169, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-E2F1 antibody (ABIN3044440)

Liu, Zhang, Lan, Wang, Mou, Shao, Liu, Yang, Lin, Lin, Ji: "GCN5 Potentiates Glioma Proliferation and Invasion via STAT3 and AKT Signaling Pathways." in: International journal of molecular sciences, Vol. 16, Issue 9, pp. 21897-910, (2016) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).

Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, (2010) (PubMed).

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: Transcription factor E2F1,E2F-1,PBR3,Retinoblastoma-associated protein 1,RBAP-1,Retinoblastoma-binding protein 3,RBBP-3,pRB-binding protein E2F-1,E2F1,RBBP3,

Tissue Specificity: All of the tissues tested and carcinomas.

Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product (pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein (pRB).

Sequence Similarities: Belongs to the E2F/DP family.

Molecular Weight: 60 kDa

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy